สารบัญ

Thai name  น้ำมันโรสแมรี (NAMMAN ROSEMARY)

Category  Pharmaceutic aid.

             Rosemary Oil is the volatile oil distilled with steam from the flowering aerial parts of Rosmarinus officinalis L. (Family Lamiaceae).

Origin of plant  Rosemary Oil-yielding plant is native to Mediterranean region, Turkey, and some eastern European countries such as Albania.   

Constituents  Rosemary Oil contains monoterpenes and their esters such as borneol, bornyl acetate, camphene, 1,8-cineole, limonene, and pinene.  It also contains bitter substances and flavonoids.

Description  Colourless to pale yellow liquid; odour aromatic, charcteristic; taste bitter and camphor-like.

Other relevant information  Oral use is not recommended for pregnant and nursing women.

Packaging and storage  Rosemary Oil shall be kept in well-filled, tightly closed containers, protected from light, and stored at a temperature not exceeding 25°.

Identification  Carry out the test as described in the “Thin-Layer Chromatography” (Appendix 3.1).
Standard solution  Dissolve 50 mg of borneol and 50 mg of bornyl acetate and 100 µL of cineole in toluene and dilute to 10 mL with the same solvent.
Test solution  Dissolve 0.5 mL of the sample in toluene and dilute to 10 mL with the same solvent.
Adsorbent  Silica gel G.
Mobile phase  Ethyl acetate and toluene (5:95).
Application  Apply 10 µL each of Standard solution and Test solution as 10-mm bands.
Development and drying  Dry the developed plate in air.
Detection  Spray the plate with vanillin-sulfuric acid TS1, heat at 105° for 10 minutes, and examine immediately in daylight.
Results  When examined in daylight, the test solution shows a violet-blue band due to borneol and a dark blue band due to cineole in the lower third of the chromatogram and a pale bluish grey band due to bornyl acetate in the middle of the chromatogram, corresponding in colour and R_f to the bands shown by the standard solution.  A violet-pink band in the middle of the chromatogram, a violet-grey band and a dark violet band in the upper third of the chromatogram are present.

Solubility test  Soluble, at 20°, in 1 part of ethanol (90 per cent).

Relative density  0.893 to 0.910 (Appendix 4.9).

Refractive index  1.464 to 1.473, at 20º (Appendix 4.7).

Optical rotation  5° to +8° (Appendix 4.8).

Acid value  Not more than 1.0 (Appendix 5.4).