สารบัญ

Category  Bitter.

        Nux Vomica Tincture contains not less than 0.15 per cent w/w and not more than 0.30 per cent w/w for the sum of brucine (C₂₃H₂₆N₂O₄) and strychnine (C₂₁H₂₂N₂O₂), of which 43 per cent to 67 per cent is strychnine.

               It may be prepared as follows.
        Nux Vomica, in No. 710 powder        1 part
        Ethanol (70 Per Cent)                     10 parts
        Mix the powdered Nux Vomica with ethanol (70 per cent) as prescribed.  Macerate for at least 10 days with sufficient shaking.  Separate the residue from the ethanol and strain under pressure if necessary.  Allow the combined liquid to stand for 48 hours and filter. 

Dose  0.6 to 2.4 mL.

Other relevant information  Taking more medication than recommended doses of nux vomica tincture may produce convulsions of all voluntary muscles due to strychnine toxicity.
See also under Nux Vomica.

Packaging and storage  Nux Vomica Tincture shall be kept in tightly closed containers, protected from light.

Identification  Complies with the test for Identification C described under Nux Vomica.

Ethanol content  60 to 70 per cent v/v (Method II, Appendix 6.5).

Assay  Carry out the determination as described in the “Liquid Chromatography” (Appendix 3.5). 
Standard preparation, Mobile phase, Chromatographic System, System Suitability, and Procedure  Proceed as directed in the Assay under Nux Vomica.
Assay preparation  Transfer about 2 g of Nux Vomica Tincture, accurately weighed, to a 20-mL volumetric flask, add sufficient amount of ethanol (60 per cent), mix, and dilute with the same solvent to volume.
Calculation  Calculate the contents of brucine and strychnine in the portion of the Tincture taken by the expression:

A_uWP /A_sw10,

in which A_u is the area of the peak due to brucine or to strychnine obtained from Assay preparation; A_s is the area of the peak due to brucine or to strychnine obtained from Standard preparation; w is the weight, in g, of Nux Vomica Tincture in Assay preparation, W is the weight, in g, of Brucine RS or of Strychnine RS in Standard preparation; P is the declared content of C₂₃H₂₆N₂O₄ in Brucine RS, or C₂₁H₂₂N₂O₂ in Strychnine RS.

Other requirements  Complies with the requirements described under “Tinctures” (Appendix 1.16).