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      Ipecacuanha Liquid Extract contains not less than 1.90 per cent and not more than 2.10 per cent of total alkaloids, calculated as emetine (C₂₉H₄₀N₂O₄).

      Ipecacuanha, in fine powder                    1000 g
        Ethanol (80 Per Cent)  a sufficient quantity  Exhaust the Ipecacuanha by percolation (Appendix 7.18) with Ethanol (80 Per Cent), reserving the first
750 mL of the percolate.  Remove the ethanol from the remainder of the percolate by evaporation under reduced pressure at a temperature not exceeding 60º and dissolve the residual extract in the reserved portion.  Determine the proportion of alkaloids in the liquid thus obtained by the Assay described below.  To the remainder of the liquid add sufficient Ethanol (80 Per Cent) to produce an Ipecacuanha Liquid Extract containing 2 per cent w/v of total alkaloids calculated as emetine.  Allow to stand for not less than 24 hours and filter. 

Description  Dark brown liquid.

Ethanol content  63 to 69 per cent  v/v (Method II, Appendix 6.5).

Relative density  0.910 to 0.960 (Appendix 4.9).

Assay  To 5.0 mL of Ipecacuanha Liquid Extract in a separator add 20 mL of water, 5 mL of 1 M sulfuric acid and 10 mL of chloroform and shake well.  Transfer the chloroform extract to a second separator containing a mixture of 4 mL of ethanol and 20 mL of 0.05 M sulfuric acid, shake, allow to separate and discard the chloroform layer.  Continue the extraction of the liquid in the first separator with two 10-mL portions of chloroform, transferring the chloroform solution each time to the second separator and washing as before.  Transfer the acidic liquid from the second separator to the first separator, make distinctly alkaline with ammonia TS and shake with successive portions of chloroform until complete extraction of the alkaloids is effected, washing each chloroform solution with the same 10 mL of water contained in a third separator.  Remove the chloroform, add to the residue 2 mL of ethanol, evaporate to dryness and dry for 5 minutes at 80° in a current of air.  Dissolve the residue in 2 mL of ethanol, previously neutralized to methyl red TS, add 10.0 mL of 0.05 M sulfuric acid VS and titrate with 0.1 M sodium hydroxide VS using methyl red-methylene blue TS as indicator.  Each mL of 0.05 M sulfuric acid VS is equivalent to 24.03 mg of total alkaloids, calculated as emetine (C₂₉H₄₀N₂O₄).

Other requirements  Complies with the requirements described under “Extracts” (Appendix 1.16).